Composite

Part:BBa_K173006:Design

Designed by: Lorenzo Pasotti, Letizia Diamante, Susanna Schiavi, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

tetR inverter - beta-galactosidase generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used BioBrick Standard Assembly.

Source

BBa_K173005, UNIPV-Pavia iGEM 2009 team;

BBa_K173004, UNIPV-Pavia iGEM 2009 team.

References

[1] Lutz R and Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res 1997 Mar 15; 25(6) 1203-10.

[2] Matthews B. "The structure of E. coli beta-galactosidase". 2005 Jun;328(6):549-56.